Human IL-17 ELISA Kit

Catalog Number: AYQ-E11153

Lead time: 3-4 business days

Please choose size: *

$0.00

Qty:

Products specifications

Background	Interleukin 17A (IL-17 or IL-17A) is a pro-inflammatory cytokine. This cytokine is produced by a group of T helper cell known as T helper 17 cell in response to their stimulation with IL-23. Originally, Th17 was identified in 1993 by Rouvier et al. who isolated IL17 transcript from a rodent T-cell hybridoma. The protein encoded by IL17A is a founding member of IL-17 family (see below). IL17 protein exhibits a high homology with a viral IL-17-like protein encoded in the genome of T-lymphotropic rhadinovirus Herpesvirus saimiri. In rodents, IL-17 is often referred to as CTLA8. The biologically active IL-17 interacts with type I cell surface receptor IL-17R. In turn, there are at least three variants of IL-17R referred to as IL17RA, IL17RB, and IL17RC. After binding to the receptor, IL-17 activates several signalling cascades that, in turn, lead to the induction of chemokines. Acting as chemoattractants, these chemokines recruit the immune cells, such as monocytes and neutrophils to the site of inflammation. Typically, the signaling events mentioned above follow an invasion of the body by pathogens. Promoting the inflammation, IL-17 acts in concert with tumor necrosis factor and interleukin-1. Moreover, an activation of IL-17 signalling is often observed in the pathogenesis of various autoimmune disorders, such as psoriasis.
Typical Data	ELISA: Human IL-17 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Applications	ELISA
Sample Volume	50-100ul
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Assay Type	Sandwich ELISA
Intra Assay	CV%<8%
Inter Assay	CV%<10%
Sensitivity	12.5 pg/ml
Standard Curve Range	15.6 pg/ml - 1000 pg/ml
Notes	Please contact our Technical Services with any questions regarding species reactivity
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Specificity	This assay has high sensitivity and excellent specificity for detection of human IL-17. No significant cross-reactivity or interference between human IL-17 and analogues was observed.
Species	Human
Synonym	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
Gene Symbol	IL-17
Gene ID	3605
Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human IL-17 ELISA Kit

Information

Customer service

My account

Follow us