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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
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Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
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Western Blot
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ELISA
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Human IL-36 gamma/IL-1F9 ELISA Kit
Human IL-36 gamma/IL-1F9 ELISA Kit
Catalog Number:
AYQ-E11135
Lead time:
3-4 business days
Please choose size:
*
96 T
96X2 T
96x5 T
$
0.00
Qty:
Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
56300
Gene Symbol
IL-36 gamma
Synonym
IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma
Species
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human IL-36 gamma/IL-1F9. No significant cross-reactivity or interference between human IL-36 gamma/IL-1F9 and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
18.75 pg/ml - 1200 pg/ml
Sensitivity
15 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Human IL-36 gamma/IL-1F9 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
Interleukin-36 gamma previously known as interleukin-1 family member 9 (IL1F9) is a protein that in humans is encoded by the IL36G gene. The protein encoded by this gene is a member of the interleukin-1 cytokine family. This gene and eight other interleukin-1 family genes form a cytokine gene cluster on chromosome 2. The activity of this cytokine is mediated via the interleukin-1 receptor-like 2 (IL1RL2/IL1R-rp2/IL-36 receptor), and is specifically inhibited by interleukin-36 receptor antagonist, (IL-36RA/IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin-1 β (IL-1β) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a multiple Pathogen-Associated Molecular Patterns (PAMPs). IL-36γ expression has been linked to psoriasis lesions and has been used as a biomarker for differentiating between eczema and psoriasis. As with many other interleukin-1 family cytokines IL-36γ requires proteolytic cleavage of its N-terminus for full biological activity. However, unlike IL-1β the activation of IL-36γ is inflammasome-independent and is specifically cleaved by the protease cathepsin S.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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