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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
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Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
Isotype Control Antibodies
Western Blot
Matched Antibody Pairs
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ELISA
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Human IL-36Ra/IL-1F5 ELISA Kit
Human IL-36Ra/IL-1F5 ELISA Kit
Catalog Number:
AYQ-E10984
Lead time:
3-4 business days
Please choose size:
*
96X1 T
96X2 T
96X5 T
$
0.00
Qty:
Product Attachments
Human IL-36Ra_IL-1F5 ELISA Kit Manual.pdf
Human IL-36Ra_IL-1F5 ELISA Kit SDS.pdf
Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
26525
Gene Symbol
IL-36RA
Synonym
FIL1 delta; IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; Interleukin 36 Receptor Antagonist; Interleukin-36 Receptor Antagonist
Species
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IL-36Ra/IL-1F5 . No significant cross-reactivity or interference between Human IL-36Ra/IL-1F5 and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
93.8--6000 pg/ml
Sensitivity
75 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Human IL-36Ra/IL-1F5 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
In humans, there are 4 distinct interleukin-36 genes. They are, three receptor agonists: IL36A, IL36B, and IL36G, and one receptor antagonist IL36RA that bind the IL-36 receptor (IL1RL2/IL-1Rrp2/IL-36 receptor) with varying affinities. The agonists are known to activate NF-κB and mitogen-activated protein kinases to induce various proinflammatory mediators. It has also been found to activate T cell proliferation and release of IL-2. Due to their predominant expression in epithelial tissues, IL-36 cytokines are believed to play a significant role in the pathogenesis of skin diseases, especially that of psoriasis.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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