Human IL-36Ra/IL-1F5 ELISA Kit

Catalog Number: AYQ-E10984
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 26525
Gene Symbol IL-36RA
Synonym FIL1 delta; IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; Interleukin 36 Receptor Antagonist; Interleukin-36 Receptor Antagonist
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human IL-36Ra/IL-1F5 . No significant cross-reactivity or interference between Human IL-36Ra/IL-1F5 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 93.8--6000 pg/ml
Sensitivity 75 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human IL-36Ra/IL-1F5 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background In humans, there are 4 distinct interleukin-36 genes. They are, three receptor agonists: IL36A, IL36B, and IL36G, and one receptor antagonist IL36RA that bind the IL-36 receptor (IL1RL2/IL-1Rrp2/IL-36 receptor) with varying affinities. The agonists are known to activate NF-κB and mitogen-activated protein kinases to induce various proinflammatory mediators. It has also been found to activate T cell proliferation and release of IL-2. Due to their predominant expression in epithelial tissues, IL-36 cytokines are believed to play a significant role in the pathogenesis of skin diseases, especially that of psoriasis.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.