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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
|
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Gene ID
|
3567 |
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Gene Symbol
|
IL-5 |
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Synonym
|
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I |
|
Species
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Human |
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Specificity
|
This assay has high sensitivity and excellent specificity for detection of human IL-5. No significant cross-reactivity or interference between human IL-5 and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
4.69 pg/ml - 300 pg/ml |
|
Sensitivity
|
3.75 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
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Typical Data
|
ELISA: Human IL-5 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Interleukin 5 (IL5) is an interleukin produced by type-2 T helper cells and mast cells. Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion. It is also a key mediator in eosinophil activation. |
