Human Kallikrein 5 ELISA Kit

Catalog Number: AYQ-E11250

Lead time: 3-4 business days

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Typical Data	ELISA: Human Kallikrein 5 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	Kallikrein-related peptidase 5 (KLK5), formerly known as stratum corneum tryptic enzyme (SCTE), is a serine protease expressed in the epidermis. In humans it is encoded by the KLK5 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. Alternative splicing results in multiple transcript variants encoding the same protein. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
Applications	ELISA
Sample Volume	50-100ul
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Assay Type	Sandwich ELISA
Intra Assay	CV%<8%
Inter Assay	CV%<10%
Sensitivity	12.5 pg/ml
Standard Curve Range	15.6 pg/ml - 1000 pg/ml
Notes	Please contact our Technical Services with any questions regarding species reactivity
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Specificity	This assay has high sensitivity and excellent specificity for detection of human Kallikrein 5. No significant cross-reactivity or interference between human Kallikrein 5 and analogues was observed.
Species	Human
Synonym	EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme
Gene Symbol	KLK5
Gene ID	25818
Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human Kallikrein 5 ELISA Kit

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