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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
|
4288 |
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Gene Symbol
|
Ki-67 |
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Synonym
|
antigen identified by monoclonal Ki-67; antigen KI-67; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-1; MKI67; PPP1R105; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Ki-67/MKI67. No significant cross-reactivity or interference between human Ki-67/MKI67 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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6.25 pg/ml - 400 pg/ml |
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Sensitivity
|
5 pg/ml |
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Inter Assay
|
CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Ki-67/MKI67 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. |
