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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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3827 |
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Gene Symbol
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KNG1 |
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Synonym
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alpha 2-thiol Proteinase Inhibitor; BDK; Kininogen; KNG1 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Kininogen. No significant cross-reactivity or interference between human Kininogen and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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125 pg/ml - 8000 pg/ml |
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Sensitivity
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100 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human Kininogen ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Kininogens are proteins that are defined by their role as precursors for kinins, but that also can have additional roles. Kinins are biologically active peptides, the parent form is bradykinin. The two main kininogen types are: High-molecular-weight kininogen, which is produced mostly by the liver but is synthesized in endothelial cells and is present in platelets and neutrophils. It acts as a cofactor for prekallikrein, factor XI, and factor XII in the coagulation and inflammation systems. It has no intrinsic enzymatic activity. These high molecular weight kininogens are cleaved into bradykinin and kallidin by tissue and plasma kallikreins. Low-molecular-weight kininogen, which is produced locally by numerous tissues, and secreted together with tissue kallikrein.
They are both spliced from the same precursor. A third type, T-kininogen, is found in rats but not humans. Closely related proteins include cystatin. |
