Human Klotho ELISA Kit

Catalog Number: AYQ-E10922
Lead time: 3-4 business days
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$0.00
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 9365
Gene Symbol KL
Synonym EC 3.2.1; EC 3.2.1.31; KL; Klotho
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human Klotho . No significant cross-reactivity or interference between Human Klotho and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 78.1--5000 pg/ml
Sensitivity 62.5 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Klotho ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Klotho is an enzyme that in humans is encoded by the KL gene. This gene encodes a type-I membrane protein that is related to β-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing, bone loss and alcohol consumption.Transgenic mice that overexpress Klotho live longer than wild-type mice.
Product tags
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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