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Bone Biomarkers
Cardiac Biomarkers
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Assay Kits
Magnetic beads
Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
Antibodies
Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
Isotype Control Antibodies
Western Blot
Matched Antibody Pairs
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ELISA
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Human Klotho ELISA Kit
Human Klotho ELISA Kit
Catalog Number:
AYQ-E10922
Lead time:
3-4 business days
Please choose size:
*
96X1 T
96X2 T
96X5 T
$
0.00
Qty:
Product Attachments
Human Klotho ELISA Kit Manual.pdf
Human Klotho ELISA Kit SDS.pdf
Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
9365
Gene Symbol
KL
Synonym
EC 3.2.1; EC 3.2.1.31; KL; Klotho
Species
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human Klotho . No significant cross-reactivity or interference between Human Klotho and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
78.1--5000 pg/ml
Sensitivity
62.5 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Human Klotho ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
Klotho is an enzyme that in humans is encoded by the KL gene. This gene encodes a type-I membrane protein that is related to β-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing, bone loss and alcohol consumption.Transgenic mice that overexpress Klotho live longer than wild-type mice.
Product tags
Klotho
(3)
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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