Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Gene ID
|
950 |
Gene Symbol
|
LIMPII |
Synonym
|
AMRF; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II); CD36 antigen; CD36L2; CD36L2LIMP II; HLGP85; LGP85; LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; LIMPII; LIMPIICD36 antigen-like 2; LPG85; lysosome membrane protein 2; Lysosome membrane protein II; SCARB2; Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein; scavenger receptor class B, member 2; SRB2; SR-B2; SR-BII |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human LIMPII/SR-B2 . No significant cross-reactivity or interference between Human LIMPII/SR-B2 and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
125--8000 pg/ml |
Sensitivity
|
100 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human LIMPII/SR-B2 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
Lysosome membrane protein 2 (LIMP-2) is a protein that in humans is encoded by the SCARB2 gene.LIMP-2 is expressed in brain, heart, liver, lung and kidney, mainly in the membrane of lysosome organelles; however, in cardiac muscle, LIMP-2 is also expressed at intercalated discs. LIMP-2 in a membrane protein in lysosomes that functions to regulate lysosomal/endosomal transport. Mutations in LIMP-2 have been shown to cause Gaucher disease, myoclonic epilepsy, and action myoclonus renal failure syndrome. Abnormal levels of LIMP-2 have also been found in patients with hypertrophic cardiomyopathy. |