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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
4153 |
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Gene Symbol
|
MBL |
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Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human MBL . No significant cross-reactivity or interference between Human MBL and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
0.625 -- 40 ng/ml |
|
Sensitivity
|
0.5 ng/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
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Typical Data
|
ELISA: Human MBL ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family
of collectins, showing calcium-dependent binding to certain sugars. It consists of oligomers of triple-chain
subunits and its binding and complement activating activities depend on its normal oligomerization. On
binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means
of its own lectin pathway, dependent on the MBL-associated serine proteases (MASPs). Because of the
presence of different structural and promoter alleles in the population, 12% or more of the population
have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum. |
