Human Meprin alpha Subunit/MEP1A ELISA Kit

Catalog Number: AYQ-E11317

Lead time: 3-4 business days

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Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID	4224
Gene Symbol	MEP1A
Synonym	bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); EC 3.4.24; EC 3.4.24.18; endopeptidase-2; MEP1A; meprin A subunit alpha; meprin A, alpha (PABA peptide hydrolase); Meprin alpha Subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA
Species	Human
Specificity	This assay has high sensitivity and excellent specificity for detection of human Meprin alpha Subunit/MEP1A. No significant cross-reactivity or interference between human Meprin alpha Subunit/MEP1A and analogues was observed.
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes	Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range	1.56 ng/ml - 100 ng/ml
Sensitivity	1.25 ng/ml
Inter Assay	CV%<10%
Intra Assay	CV%<8%
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume	50-100ul
Applications	ELISA
Typical Data	ELISA: Human Meprin alpha Subunit/MEP1A ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	Meprin A subunit alpha also known as endopeptidase-2 or PABA peptide hydrolase is the alpha subunit of the meprin A enzyme that in humans is encoded by the MEP1A gene. The MEP1A locus is on chromosome 6p in humans and on chromosome 17 in mice. The meprin alpha subunit product of the MEP1A gene is processed in the endoplasmic reticulum during intracellular transport, and is secreted as homomeric meprin A. Meprin alpha subunits may self-associate, and once secreted, form very large multimers, with a molecular mass of over 1 million daltons. In cells concurrently expressing MEP1B, the meprin alpha and meprin beta subunits form disulfide dimers that interact to form membrane bound heterotetrameric meprin A.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human Meprin alpha Subunit/MEP1A ELISA Kit

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