|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
|
Gene ID
|
5562 |
|
Gene Symbol
|
AMPK |
|
Synonym
|
AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK alpha 1; AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK subunit alpha-1; AMPK; AMPK1; AMPKa1; EC 2.7.11; EC 2.7.11.1; MGC33776; MGC57364; PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit |
|
Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Phospho-AMPK alpha 1 (T183). No significant cross-reactivity or interference between Human Phospho-AMPK alpha 1 (T183) and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
62.5-4000 pg/ml |
|
Sensitivity
|
50 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human Phospho-AMPK alpha 1 (T183) ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene.The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
