Human Phospho-Chk2 (T68) ELISA Kit

Catalog Number: AYQ-E10886

Lead time: 3-4 business days

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Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID	11200
Gene Symbol	CHK2
Synonym	CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog
Species	Human
Specificity	This assay has high sensitivity and excellent specificity for detection of Human Phospho-Chk2 (T68) . No significant cross-reactivity or interference between Human Phospho-Chk2 (T68) and analogues was observed.
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes	Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range	0.313--20 ng/ml
Sensitivity	0.25 ng/ml
Inter Assay	CV%<10%
Intra Assay	CV%<8%
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume	50-100ul
Applications	ELISA
Typical Data	ELISA: Human Phospho-Chk2 (T68) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	The CHEK2 gene encodes for checkpoint kinase 2 (CHK2), a protein that acts a tumor suppressor. CHK2 regulates cell division, and has the ability to prevent cells from dividing too rapidly or in an uncontrolled manner.When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2. Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs). Thus, CHK2’s inhibition of the CDC25 phosphatases prevents entry of the cell into mitosis. Furthermore, the CHK2 protein interacts with several other proteins including p53 (p53). Stabilization of p53 by CHK2 leads to cell cycle arrest in phase G1. Furthermore, CHK2 is known to phosphorylate the cell-cycle transcription factor E2F1 and the promyelocytic leukemia protein (PML) involved in apoptosis.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human Phospho-Chk2 (T68) ELISA Kit

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