Human Phospho-EphB4 ELISA Kit

Catalog Number: AYQ-E10271

Lead time: 3-4 business days

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Background	Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene.Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development
Typical Data	ELISA: Human Phospho-EphB4ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Applications	ELISA
Sample Volume	50-100ul
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Assay Type	Sandwich ELISA
Intra Assay	CV%<8%
Inter Assay	CV%<10%
Notes	Please contact our Technical Services with any questions regarding species reactivity
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Specificity	This assay has high sensitivity and excellent specificity for detection Human Phospho-EphB4. No significant cross-reactivity or Human Phospho-EphB4 and analogues was observed.
Species	Human
Synonym	EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11
Gene Symbol	EphB4
Gene ID	2050
Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human Phospho-EphB4 ELISA Kit

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