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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
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Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
Isotype Control Antibodies
Western Blot
Matched Antibody Pairs
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ELISA
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Human Phospho-JNK2 (T183/Y185) ELISA Kit
Human Phospho-JNK2 (T183/Y185) ELISA Kit
Catalog Number:
AYQ-E10086
Lead time:
3-4 business days
Please choose size:
*
96X1 T
96X2 T
96X5 T
$
0.00
Qty:
Product Attachments
Human Phospho-JNK2 (T183/Y185) ELISA Kit Manual.pdf
Human Phospho-JNK2 (T183/Y185) ELISA Kit SDS.pdf
Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
5601
Gene Symbol
JNK2
Synonym
c-Jun kinase 2; c-Jun N-terminal kinase 2; EC 2.7.11; EC 2.7.11.24; JNK2; JNK2A; JNK2BETA; JNK2JNK2ALPHA; JNK-55; Jun kinase; MAP kinase 9; MAPK 9; MAPK9; mitogen-activated protein kinase 9; p54a; p54aSAPK; PRKM9JNK2B; SAPK; SAPK1 alpha; Stress-activated protein kinase JNK2
Species
Human
Specificity
This assay has high sensitivity and excellent specificity for detection Human Phospho-JNK2 (T183/Y185). No significant cross-reactivity or interference between Human Phospho-JNK2 (T183/Y185)and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
156.25--10000 pg/ml
Sensitivity
125 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Human Phospho-JNK2 (T183/Y185) ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
JNK2 a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses including UV radiation. Phosphorylates specific transcription factors such as c-Jun and ATF2, mediating immediate-early gene expression. Closely related to JNK1. Both are involved in UV radiation induced apoptosis. Blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells in the Mouse
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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