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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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65125 |
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Gene Symbol
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WNK1 |
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Synonym
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EC 2.7.11; EC 2.7.11.1; Erythrocyte 65 kDa protein; HSAN2hereditary sensory neuropathy, type II; HSN2MGC163339; hWNK1; KDP; KDPMGC163341; KIAA0344; Kinase deficient protein; p65; PHA2C; PRKWNK1; PRKWNK1PSK; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; serine/threonine-protein kinase WNK1; WNK lysine deficient protein kinase 1; WNK1 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Human Phospho-WNK1 (T60) . No significant cross-reactivity or interference between Human Phospho-WNK1 (T60) and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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15.6--1000 pg/ml |
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Sensitivity
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12.5 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human Phospho-WNK1 (T60) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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WNK (lysine deficient protein kinase 1), also known as WNK1, is an enzyme that is encoded by the WNK1 gene. WNK1 is serine-threonine kinase and part of the "with no lysine/K" kinase WNK family. The predominant role of WNK1 is the regulation of cation-Cl− cotransporters (CCCs) such as the sodium chloride cotransporter (NCC), basolateral Na-K-Cl symporter (NKCC1), and potassium chloride cotransporter (KCC1) located within the kidney. CCCs mediate ion homeostasis and modulate blood pressure by transporting ions in and out of the cell.[5] WNK1 mutations as a result have been implicated in blood pressure disorders/diseases; a prime example being familial hyperkalemic hypertension (FHHt). |
