Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Gene ID
|
65125 |
Gene Symbol
|
WNK1 |
Synonym
|
EC 2.7.11; EC 2.7.11.1; Erythrocyte 65 kDa protein; HSAN2hereditary sensory neuropathy, type II; HSN2MGC163339; hWNK1; KDP; KDPMGC163341; KIAA0344; Kinase deficient protein; p65; PHA2C; PRKWNK1; PRKWNK1PSK; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; serine/threonine-protein kinase WNK1; WNK lysine deficient protein kinase 1; WNK1 |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Phospho-WNK1 (T60) . No significant cross-reactivity or interference between Human Phospho-WNK1 (T60) and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
15.6--1000 pg/ml |
Sensitivity
|
12.5 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human Phospho-WNK1 (T60) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
WNK (lysine deficient protein kinase 1), also known as WNK1, is an enzyme that is encoded by the WNK1 gene. WNK1 is serine-threonine kinase and part of the "with no lysine/K" kinase WNK family. The predominant role of WNK1 is the regulation of cation-Cl− cotransporters (CCCs) such as the sodium chloride cotransporter (NCC), basolateral Na-K-Cl symporter (NKCC1), and potassium chloride cotransporter (KCC1) located within the kidney. CCCs mediate ion homeostasis and modulate blood pressure by transporting ions in and out of the cell.[5] WNK1 mutations as a result have been implicated in blood pressure disorders/diseases; a prime example being familial hyperkalemic hypertension (FHHt). |