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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
7525 |
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Gene Symbol
|
YES |
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Synonym
|
cellular yes-1 protein; c-yes; EC 2.7.10; EC 2.7.10.2; HsT441; P61-YES; Proto-oncogene c-Yes; proto-oncogene tyrosine-protein kinase Yes; v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; Yamaguchi sarcoma oncogene; Yes; YES1 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for Human Phospho-Yes (Y426). No significant cross-reactivity or Human Phospho-Yes (Y426) and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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17.1875--1100 pg/ml |
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Sensitivity
|
13.75 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Phospho-Yes (Y426) ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Proto-oncogene tyrosine-protein kinase Yes is an enzyme that in humans is encoded by the YES1 gene.This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. |
