Human Pro-Gastrin-releasing Peptide ELISA Kit

Catalog Number: AYQ-E11113
Lead time: 3-4 business days
*
$0.00
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 2922
Gene Symbol GRP10
Synonym BN; Bombesin; Gastrinreleasing Peptide; Gastrin-releasing Peptide; GRP; GRP10; GRP-10; neuromedin C; pre-progastrin releasing peptide; preproGRP; proGRP
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of human Pro-Gastrin-releasing Peptide. No significant cross-reactivity or interference between human Pro-Gastrin-releasing Peptide and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 9.38 pg/ml - 600 pg/ml
Sensitivity 7.5 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Pro-Gastrin-releasing Peptide ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Gastrin-releasing peptide, also known as GRP, is a neuropeptide, a regulatory molecule that has been implicated in a number of physiological and pathophysiological processes. Most notably, GRP stimulates the release of Gastrin from the G cells of the stomach. The gene from which GRP is derived encodes a number of bombesin-like peptides. Its 148-amino acid preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27-amino acid gastrin-releasing peptide or the 10-amino acid neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation.