|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
|
|
Gene ID
|
2896 |
|
Gene Symbol
|
Progranulin |
|
Synonym
|
Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin |
|
Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of human Progranulin. No significant cross-reactivity or interference between human Progranulin and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
62.5 pg/ml - 4000 pg/ml |
|
Sensitivity
|
50 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human Progranulin ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Progranulin produces a variety of active 6 kDa granulin peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Elastase, proteinase 3 and matrix metalloproteinase are proteases capable of cleaving progranulin into individual granulin peptides. While progranulin is associated with anti-inflammation, cleaved granulin peptides have been implicated in pro-inflammatory behavior. A showed that granulin peptides may also participate in toxic activity in C. elegans. |
