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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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5972 |
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Gene Symbol
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Renin |
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Synonym
|
angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; REN; renin precursor, renal; Renin |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Renin. No significant cross-reactivity or interference between human Renin and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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62.5 pg/ml - 4000 pg/ml |
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Sensitivity
|
50 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Renin ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Renin (etymology and pronunciation), also known as an angiotensinogenase, is an aspartic protease protein and enzyme secreted by the kidneys that participates in the body's renin–angiotensin–aldosterone system (RAAS)—also known as the renin–angiotensin–aldosterone axis—that mediates the volume of extracellular fluid (blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. Thus, it regulates the body's mean arterial blood pressure. Renin can be referred to as a hormone even though it has no peripheral receptors and rather has an enzymatic activity with which it hydrolyzes angiotensinogen to angiotensin I. |
