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Bone Biomarkers
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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
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Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
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ELISA
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Human sFRP-3 ELISA Kit
Human sFRP-3 ELISA Kit
Catalog Number:
AYQ-E11040
Lead time:
3-4 business days
Please choose size:
*
96X1 T
96X2 T
96X5 T
$
0.00
Qty:
Product Attachments
Human sFRP-3 ELISA Kit Manual.pdf
Human sFRP-3 ELISA Kit SDS.pdf
Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
2487
Gene Symbol
SFRP3
Synonym
FIZ; FREOS1; Frezzled; Fritz; Frizzled-related protein 1; frizzled-related protein; FRP; FRP-3; FRZB; Frzb1; FRZB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; secreted frizzled-related protein 3; sFRP3; sFRP-3; SFRP3frezzled; SRFP3frizzled homolog-related
Species
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human sFRP-3 . No significant cross-reactivity or interference between Human sFRP-3 and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
250--8000 pg/ml
Sensitivity
200 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Human sFRP-3 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
Secreted Frizzled related proteins are secreted glycoproteins with homology to the Wnt-binding domain of the Frizzled family of transmembrane receptors. sFRPs contain an N-terminal Frizzled-like cysteine-rich domain (CRD) with 10 conserved cysteines and a C-terminal heparin-binding region. sFRPs function as soluble antagonists of Wnt signaling by binding directly to Wnt proteins and preventing their interactions with Frizzled receptors.
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Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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