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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
581 |
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Gene Symbol
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Bax-alpha |
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Synonym
|
Baxalpha; Bax-alpha |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Human Total Bax-alpha . No significant cross-reactivity or interference between Human Total Bax-alpha and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
0.313--20 ng/ml |
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Sensitivity
|
0.25 ng/ml |
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Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Total Bax-alpha ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
B cell leukemia/lymphoma-2 associated X protein (Bax) is a pro-apoptotic member of the Bcl-2 family of proteins found in many cell types. Bax is a cytosolic protein that translocates to the mitochondria and participates in Cytochrome c release in response to apoptotic stimuli. Aberrant expression of Bcl-2 family members can inappropriately promote or prevent apoptosis. Increased expression of Bax is associated with the apoptotic loss of neurons in Parkinson's disease. |
