Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Gene ID
|
581 |
Gene Symbol
|
Bax-alpha |
Synonym
|
Baxalpha; Bax-alpha |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Total Bax-alpha . No significant cross-reactivity or interference between Human Total Bax-alpha and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
0.313--20 ng/ml |
Sensitivity
|
0.25 ng/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human Total Bax-alpha ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
B cell leukemia/lymphoma-2 associated X protein (Bax) is a pro-apoptotic member of the Bcl-2 family of proteins found in many cell types. Bax is a cytosolic protein that translocates to the mitochondria and participates in Cytochrome c release in response to apoptotic stimuli. Aberrant expression of Bcl-2 family members can inappropriately promote or prevent apoptosis. Increased expression of Bax is associated with the apoptotic loss of neurons in Parkinson's disease. |