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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
51738 |
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Gene Symbol
|
GHRL |
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Species
|
Human |
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Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Total Ghrelin . No significant cross-reactivity or interference between Human Total Ghrelin and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
156.25 -- 10000 pg/ml |
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Sensitivity
|
125 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Total Ghrelin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced
by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are
produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is
acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and
increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma
responses of total ghrelin to food may reflect those of active ghrelin. |
