Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Gene ID
|
1026 |
Gene Symbol
|
CDKN1A |
Synonym
|
CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1 |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Total p21/CIP1/CDKN1A . No significant cross-reactivity or interference between Human Total p21/CIP1/CDKN1A and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
62.5--4000 pg/ml |
Sensitivity
|
50 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human Total p21/CIP1/CDKN1A ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
p21/Cip1 (alternatively p21Waf1), also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a cyclin-dependent kinase inhibitor (CKI) that is capable of inhibiting all cyclin/CDK complexes ,though is primarily associated with inhibition of CDK2. p21 represents a major target of p53 activity and thus is associated with linking DNA damage to cell cycle arrest. This protein is encoded by the CDKN1A gene located on chromosome 6 (6p21.2) in humans |