Human Total PON1 ELISA Kit

Catalog Number: AYQ-E10875
Lead time: 3-4 business days
Products specifications
Background Serum paraoxonase/arylesterase 1 (PON1) also known as A esterase , homocysteine thiolactonase or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. Paraoxonase 1 has esterase and more specifically paraoxonase activity. Serum PON1 is found in all mammalian species studied so far but is not present in the serum of birds, fish and reptiles or in insects. PON1 is the first discovered member of a multigene family also containing PON2 and PON3, the genes for which are located adjacent to each other on chromosome 7.
Typical Data ELISA: Human Total PON1 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Applications ELISA
Sample Volume 50-100ul
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Assay Type Sandwich ELISA
Intra Assay CV%<8%
Inter Assay CV%<10%
Sensitivity 125 pg/ml
Standard Curve Range 156--10000 pg/ml
Notes Please contact our Technical Services with any questions regarding species reactivity
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Specificity This assay has high sensitivity and excellent specificity for detection of Human Total PON1 . No significant cross-reactivity or interference between Human Total PON1 and analogues was observed.
Species Human
Synonym A-esterase 1; Aromatic esterase 1; arylesterase B-type; EC; EC; EC; ESA; ESAesterase A; K-45; MVCD5; paraoxonase 1; PON 1; PON1; PONparaoxonase B-type; serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1
Gene Symbol PON1
Gene ID 5444
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.