Human TWEAK/TNFSF12 ELISA Kit

Catalog Number: AYQ-E10979

Lead time: 3-4 business days

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Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID	8742
Gene Symbol	TNFSF12
Synonym	APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK
Species	Human
Specificity	This assay has high sensitivity and excellent specificity for detection of Human TWEAK/TNFSF12 . No significant cross-reactivity or interference between Human TWEAK/TNFSF12 and analogues was observed.
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes	Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range	62.5--4000 pg/ml
Sensitivity	50 pg/ml
Inter Assay	CV%<10%
Intra Assay	CV%<8%
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume	50-100ul
Applications	ELISA
Typical Data	ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	TWEAK was discovered in 1997. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. Leukocytes are the main source of TWEAK including human resting and activated monocytes, dendritic cells and natural killer cells. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human TWEAK/TNFSF12 ELISA Kit

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