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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
7314 |
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Gene Symbol
|
UBC |
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Synonym
|
FLJ25987; MGC8385; polyubiquitin B; polyubiquitin-B; RPS27A; UBA52; UBC; ubiquitin B; Ubiquitin+1 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Human Ubiquitin+1. No significant cross-reactivity or Human Ubiquitin+1and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
125--8000 pg/ml |
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Sensitivity
|
100 pg/ml |
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Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
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ELISA: Human Ubiquitin+1 ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Ubiquitin is a small protein that exists in all eukaryotic cells. It performs its myriad functions through conjugation to a large range of target proteins. A variety of different modifications can occur. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 lysine residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity. |
