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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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8840 |
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Gene Symbol
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WISP-1 |
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Synonym
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CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Human WISP-1/CCN4 . No significant cross-reactivity or interference between Human WISP-1/CCN4 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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31.3--2000 pg/ml |
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Sensitivity
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25 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human WISP-1/CCN4 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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WISP-1 promotes mesenchymal cell proliferation and osteoblastic differentiation, and represses chondrocytic differentiation. WISP-1 binds BMP2 and enhances BMP2 function in osteogenesis. These activities may be modulated by its direct binding to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue |
