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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
6340 |
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Gene Symbol
|
SCNN1G |
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Species
|
Human |
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Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human yENaC . No significant cross-reactivity or interference between Human yENaC and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
0.4 -- 9.4 ng/ml |
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Sensitivity
|
0.32 ng/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human yENaC ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and
normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the -subunit and putative
release of a 43-amino acid inhibitory tract from the -subunit ectodomain. |
