Click Here for Manual
This matched Ab set contains the capture and detection antibodies required for the development of sandwich immunoassays to detect/ quantify IL-3 in serum/ cell culture supernatant.
Optional: Biotinylated detection antibody
Applications: ELISA, IP-WB, FRET/TR-FRET, AlphaLISA/AlphaScreen, ultrasensitive PLA(proximity ligation assay), cell- based RCA (rolling circle amplification), Luminex and other bead-based assays
Assay Range: 125 pg/ml to 8000 pg/mL (as determined by sandwich ELISA using cell lysates)
Kit Contents: Reagents are sufficient for atleast 5 x 96-well plates
Capture Antibody: Mouse anti-human IL-3 (50 μg)
Detection Antibody: Rabbit anti-human IL-3 (5 μg)