Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Influenza A . No significant cross-reactivity or interference between Human Influenza A and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human Influenza A ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to
modify their antigenicity and avoid elimination by immunity to previous generations of virus. The
nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa
subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle. |