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Bone Biomarkers
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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
Antibodies
Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
Isotype Control Antibodies
Western Blot
Matched Antibody Pairs
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ELISA
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Mouse Total Akt1 ELISA Kit
Mouse Total Akt1 ELISA Kit
Catalog Number:
AYQ-E10092
Lead time:
3-4 business days
Please choose size:
*
96X1 T
96X2 T
96X5 T
$
0.00
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Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
11651
Gene Symbol
AKT
Synonym
AKT; Akt1; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKBMGC99656; PRKBA; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; v-akt murine thymoma viral oncogene homolog 1
Species
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse Total Akt1. No significant cross-reactivity or interference between Mouse Total Akt1 and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
312.5--20000 pg/ml
Sensitivity
250 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Mouse Total Akt1 ELISA Kit (Colorimetric These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) domains. It is commonly referred to as PKB, or by both names as "Akt/PKB".
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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