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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
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Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
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ELISA
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Mouse Total GAPDH ELISA Kit
Mouse Total GAPDH ELISA Kit
Catalog Number:
AYQ-E10353
Lead time:
3-4 business days
Please choose size:
*
96X1 T
96X2 T
96X5 T
$
0.00
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Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
14433
Gene Symbol
GAPDH
Synonym
aging-associated gene 9 protein; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PDH; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC88685
Species
Mouse
Specificity
This assay has high sensitivity and excellent specificity for Mouse Total GAPDH. No significant cross-reactivity or Mouse Total GAPDH and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
156.25--10000 pg/ml
Sensitivity
125 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Mouse Total GAPDH ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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