|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
|
Gene ID
|
100144493 |
|
Gene Symbol
|
NT3 |
|
Synonym
|
3-nucleotidase; NT3; NT-3; NTF3 |
|
Species
|
Porcine |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Porcine NT-3. No significant cross-reactivity or interference Porcine NT-3 and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
31.3--2000 pg/ml |
|
Sensitivity
|
25 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Porcine NT-3 ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Neurotrophin-3 is a protein that in humans is encoded by the NTF3 gene.The protein encoded by this gene, NT-3, is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. NT-3 was the third neurotrophic factor to be characterized, after nerve growth factor (NGF) and BDNF (Brain Derived Neurotrophic Factor |
