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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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100126861 |
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Gene Symbol
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AKT |
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Synonym
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AKT; Akt1; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKBMGC99656; PRKBA; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; v-akt murine thymoma viral oncogene homolog 1 |
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Species
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Porcine |
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Specificity
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This assay has high sensitivity and excellent specificity for detection Porcine Phospho-Akt1 (S473). No significant cross-reactivity or interference between Porcine Phospho-Akt1 (S473) and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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0.625--40 ng/ml |
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Sensitivity
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0.5 ng/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Porcine Phospho-Akt1 (S473) ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) domains. It is commonly referred to as PKB, or by both names as "Akt/PKB". |
