Porcine TACE/ADAM17 ELISA Kit

Catalog Number: AYQ-E10306

Lead time: 3-4 business days

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Background	ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance. ADAM17 was the first 'sheddase' to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes.
Typical Data	ELISA: Porcine TACE/ADAM17 ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Applications	ELISA
Sample Volume	50-100ul
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Assay Type	Sandwich ELISA
Intra Assay	CV%<8%
Inter Assay	CV%<10%
Sensitivity	125 pg/ml
Standard Curve Range	156.25--10000 pg/ml
Notes	Please contact our Technical Services with any questions regarding species reactivity
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Specificity	This assay has high sensitivity and excellent specificity for detection Porcine TACE/ADAM17 . No significant cross-reactivity or Porcine TACE/ADAM17 and analogues was observed.
Species	Porcine
Synonym	ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme
Gene Symbol	TACE
Gene ID	397343
Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Porcine TACE/ADAM17 ELISA Kit

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