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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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397484 |
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Gene Symbol
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TGFA |
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Synonym
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protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha |
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Species
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Porcine |
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Specificity
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This assay has high sensitivity and excellent specificity for Porcine TGF-alpha. No significant cross-reactivity or Porcine TGF-alpha and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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7.81--500 pg/ml |
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Sensitivity
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6.25 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Porcine TGF-alpha ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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Transforming growth factor alpha (TGF-α) is a protein that in humans is encoded by the TGFA gene. As a member of the epidermal growth factor (EGF) family, TGF-α is a mitogenic polypeptide.The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling.TGF-α is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate |
