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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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25578 |
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Gene Symbol
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14-3-3 ZETA |
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Synonym
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1433 zeta; 14-3-3 zeta; KCIP-1; KCIP-1MGC126532,14-3-3-zeta; MGC111427; MGC138156; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide; YWHAZ; zeta polypeptide |
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Species
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Rat |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Rat 14-3-3 zeta . No significant cross-reactivity or interference between Rat 14-3-3 zeta and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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125--8000 pg/ml |
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Sensitivity
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100 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
|
ELISA |
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Typical Data
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ELISA: Rat 14-3-3 zeta ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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14-3-3 proteins are a family of conserved regulatory molecules that are expressed in all eukaryotic cells. 14-3-3 proteins have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 200 signaling proteins have been reported as 14-3-3 ligands. |
