Rat GIT1 ELISA Kit

Catalog Number: AYQ-E10590

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Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID	83709
Gene Symbol	GIT1
Synonym	ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT1; CAT-1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GRK-interacting protein 1
Species	Rat
Specificity	This assay has high sensitivity and excellent specificity for detection of rat GIT1. No significant cross-reactivity or interference between rat GIT1 and analogues was observed.
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes	Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range	31.3 pg/ml - 2000 pg/ml
Sensitivity	25 pg/ml
Inter Assay	CV%<10%
Intra Assay	CV%<8%
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume	50-100ul
Applications	ELISA
Typical Data	ELISA: Rat GIT1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. GIT1 contains an ARFGAP domain, Anykrin repeats, and a GRK-interacting domain. The Arf-GAP domain, which enables it to act as a GTPase activating protein (GAP) for the Arf family of GTPases, has been shown to be involved in phosphorylation and inhibition of the ADRB2. If synaptic localization of GIT1 is disturbed, then this is known to affect dendritic spine morphology and formation---this is thought to occur through the Rac1/PAK1/LIMK/CFL1 pathway.

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GIT1 (5)

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Rat GIT1 ELISA Kit

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