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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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24505 |
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Gene Symbol
|
INS |
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Synonym
|
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin |
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Species
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Rat |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of rat Insulin. No significant cross-reactivity or interference between rat Insulin and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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15.6 pg/ml - 1000 pg/ml |
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Sensitivity
|
12.5 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Rat Insulin ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Insulin (from Latin insula, island) is a peptide hormone produced by beta cells of the pancreatic islets, and it is considered to be the main anabolic hormone of the body. It regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of, especially, glucose from the blood into liver, fat and skeletal muscle cells. In these tissues the absorbed glucose is converted into either glycogen via glycogenesis or fats (triglycerides) via lipogenesis, or, in the case of the liver, into both. Glucose production and secretion by the liver is strongly inhibited by high concentrations of insulin in the blood. Circulating insulin also affects the synthesis of proteins in a wide variety of tissues. It is therefore an anabolic hormone, promoting the conversion of small molecules in the blood into large molecules inside the cells. Low insulin levels in the blood have the opposite effect by promoting widespread catabolism, especially of reserve body fat. |
