XRCC1 Antibody

Catalog Number: ASA-B1999
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

X-ray repair complementing defective repair in Chinese hamster cells 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1(15-34aa QDSTHCAENLLKADTYRKWR), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

XRCC1

Protein

DNA repair protein XRCC1

Uniprot ID

P18887

Function

Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

Tissue Specificity

Sub-cellular localization

Nucleus.

Sequence Similarities

Contains 2 BRCT domains.

Aliases

DNA repair protein XRCC1 antibody|RCC antibody|X ray repair complementing defective repair in chinese hamster antibody|X ray repair complementing defective repair in chinese hamster cells 1 antibody|X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody|X ray repair cross complementing protein 1 antibody|X ray repair, complementing defective, repair in Chinese hamster antibody|X-ray repair cross-complementing protein 1 antibody|XRCC 1 antibody|Xrcc1 antibody|XRCC1_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human Mouse, RatAssaySolution's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-XRCC1 antibody, ASA-B1999, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: HELA Cell Lysate
Anti-XRCC1 antibody, ASA-B1999, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-XRCC1 antibody, ASA-B1999, ICC
ICC: A549 Cell
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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