Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
5-hydroxytryptamine(serotonin) receptor 1A, G protein-coupled |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor(404-422aa NKDFQNAFKKIIKCKFCRQ), different from the related rat and mouse sequences by one amino acid. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
HTR1A |
Protein
|
5-hydroxytryptamine receptor 1A(5-HT-1A/5-HT1A) |
Uniprot ID
|
P08908 |
Function
|
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5- hydroxytryptamine release and in the regulation of dopamine and 5- hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli. |
Tissue Specificity
|
Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells. |
Sub-cellular localization
|
Cell membrane. |
Sequence Similarities
|
|
Aliases
|
5 HT 1A antibody|5 HT receptor 1A antibody|5 HT1A antibody|5 HT1A receptor antibody|5 hydroxytryptamine(serotonin) receptor 1A antibody|5 hydroxytryptamine(serotonin) receptor 1A G protein coupled antibody|5 hydroxytryptamine receptor 1A antibody|5-HT-1A antibody|5-HT1A antibody|5-hydroxytryptamine receptor 1A antibody|5HT 1A antibody|5HT 1A receptor antibody|5HT receptor 1A antibody|5HT1a antibody|5HT1A_HUMAN antibody|ADRB2RL1 antibody|ADRBRL1 antibody|G 21 antibody|G protein coupled receptor antibody|G-21 antibody|Gpcr 18 antibody|Gpcr18 antibody|Guanine nucleotide binding regulatory protein-coupled receptor antibody|HTR1A antibody|PFMCD antibody|Serotonin 1A receptor antibody|Serotonin 5HT1A receptor antibody|Serotonin receptor 1A antibody |
Application Details
Application |
Concentration* |
Species |
Validated Using** |
Western blot |
0.1-0.5μg/ml |
Human, Rat Mouse |
AssaySolution's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Rat Human, Mouse |
AssaySolution's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-5HT1A Receptor antibody, ASA-B0005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Human U87 Cell Lysate
Anti-5HT1A Receptor antibody, ASA-B0005, IHC(P)
Rat Brain Tissue