Western Blot

Western blotting, also known as immunoblotting is a widely used laboratory technique to separate and identify proteins, based on antigen-antibody interaction. The extract containing complex mixture of proteins is run on a gel electrophoresis and then transferred to a PVDF/ Nitrocellulose membrane. The proteins on the membrane are detected by the addition of appropriate specific antibodies and visualized as a band on blotting membrane, X-ray film, or an imaging system, depending on the type of detection system chosen. All our antibodies for western blotting went through a vigorous validation process.