Actin Antibody

Catalog Number: ASA-B0031

Lead time: 1 week

$299.00

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
actin, alpha 1, skeletal muscl	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	ACTA1
Protein	Actin, alpha skeletal muscle
Uniprot ID	P68133
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Tissue Specificity
Sub-cellular localization	Cytoplasm, cytoskeleton.
Sequence Similarities
Aliases	ACTA antibody\|ACTA1 antibody\|Actin alpha skeletal muscle antibody\|Actin antibody\|actin, alpha 1, skeletal muscle 1 antibody\|actin, alpha 1, skeletal muscle antibody\|Actin, alpha skeletal muscle antibody\|actina antibody\|actine antibody\| ACTS_HUMAN antibody\|aktin antibody\|Alpha Actin 1 antibody\|Alpha skeletal muscle Actin antibody\|alpha skeletal muscle antibody\|alpha-actin antibody\|Alpha-actin-1 antibody\|ASMA antibody\|CFTD antibody\| CFTD1antibody\|CFTDM antibody\| MPFD antibody\|NEM1 antibody\|NEM2 antibody\|NEM3 antibody\| nemaline myopathy type 3 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- ACTIN antibody, ASA-B0031, Western blotting
All lanes: Anti ACTIN (ASA-B0031) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted band size: 42KD
Observed band size: 42KD

Anti- ACTIN antibody, ASA-B0031, IHC(P)
Mouse Intestine Tissue

Anti- ACTIN antibody, ASA-B0031, IHC(P)
Rat Spleen Tissue

Actin Antibody

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification

Immunogen

Properties

Form

Size

Contents

Concentration

Storage

Additional Information Regarding the Antigen

Gene

Protein

Uniprot ID

Function

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

Application Details