ADAM10 Antibody

Catalog Number: ASA-B0032
Lead time: 1 week
$299.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ADAM metallopeptidase domain 10 Polyclonal IgG Rabbit Human WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM10(612-627aa SVQWSRHFSGRTITLQ).

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ADAM10

Protein

Disintegrin and metalloproteinase domain-containing protein 10(ADAM 10)

Uniprot ID

O14672

Function

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-

Tissue Specificity

Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Sub-cellular localization

Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Note: Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.

Sequence Similarities

Contains 1 disintegrin domain.

Aliases

A disintegrin and metalloprotease domain 10 antibody|A disintegrin and metalloproteinase domain 10 antibody|AD 10 antibody|AD10 antibody|ADA10_HUMAN antibody|ADAM 10 antibody|ADAM metallopeptidase domain 10 antibody|ADAM10 antibody|CD 156c antibody|CD156c antibody|CD156c antigen antibody|CDw156 antibody|disintegrin and metalloproteinase domain containing protein 10 antibody|Disintegrin and metalloproteinase domain-containing protein 10 antibody|HsT 18717 antibody|HsT18717 antibody|Kuz antibody|Kuzbanian antibody|Kuzbanian protein homolog antibody|MADM antibody|Mammalian disintegrin metalloprotease antibody|Mammalian disintegrin-metalloprotease antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-ADAM10 antibody, ASA-B0032, Western blotting
Lane 1: Recombinant Human ADAM10 Protein 10ng
Lane 2: Recombinant Human ADAM10 Protein 5ng
Lane 3: Recombinant Human ADAM10 Protein 2.5ng