Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| alpha-fetoprotein |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
AFP |
Protein
|
Alpha-fetoprotein |
Uniprot ID
|
P02771 |
Function
|
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
Tissue Specificity
|
Plasma. Synthesized by the fetal liver and yolk sac. |
Sub-cellular localization
|
Secreted. |
Sequence Similarities
|
Belongs to the ALB/AFP/VDB family. |
Aliases
|
AFP antibody|Alpha fetoglobulin antibody|Alpha fetoprotein antibody|Alpha fetoproteinprecursor antibody|Alpha-1-fetoprotein antibody|Alpha-fetoglobulin antibody|Alpha-fetoprotein antibody|alpha-fetoprotein, Hereditary persistence of, included antibody|FETA antibody|FETA_HUMAN antibody|Hereditary persistence of alpha fetoprotein antibody|HPAFP antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Mouse, Rat |
AssaySolution's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human |
AssaySolution's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-AFP antibody, ASA-B0048 Western blotting
All lanes: Anti AFP (ASA-B0048) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted band size: 69KD
Observed band size: 69KD
