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Product Name
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AntiCD8 alpha antibody (polyclonal)
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Pack Size
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100μg/vial
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Immunogen
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Human CD8 alpha recombinant protein (amino acids: 22-187).
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Host
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Rabbit
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Isotype
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IgG
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Reactivity
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Human
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Specificity
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No cross reactivity with other proteins
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Purification
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Immunogen affinity purified.
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Form*
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Lyophilized powder containg 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
*carrier free antibody available upon request.
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Reconstitution
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Reconstitutiing with 1 ml of distilled water will yield a concentration of 100 µg/mL.
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Application
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Western blot 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section) 0.5-1μg/ml
Note: The optimal working dilution should be determined by the end user.
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Storage
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Store at -20°C for one year. After reconstitution, sotre at 4°C for one month. store at -20˚C for a longer time. Aliquot to avoid repeated freezing and thawing.
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Alternate Names
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T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a
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Anti- CD 8 alpha antibody, A0214B, IHC(P)
Human Tonsil Tissue
Anti- CD 8 alpha antibody, A0214B, Western blotting
All lanes: Anti CD 8 alpha (A0214B) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: HL-60 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted band size: 26KD
Observed band size: 55KD
