Beclin 1 Antibody

Catalog Number: ASA-B0216

Lead time: 1 week

$339.25

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
beclin 1, autophagy related	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	BECN1
Protein	Beclin-1
Uniprot ID	Q14457
Function	Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Tissue Specificity	Ubiquitous.
Sub-cellular localization	Cytoplasm.
Sequence Similarities
Aliases	APG6 antibody\|ATG 6 antibody\|ATG6 antibody\|ATG6 autophagy related 6 homolog antibody\|Bcl-2-interacting protein beclin antibody\|Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody\|Beclin 1 autophagy related antibody\|Beclin-1 antibody\|Beclin1 antibody\|BECN 1 antibody\|BECN1 antibody\|BECN1_HUMAN antibody\|Coiled coil myosin like BCL2 interacting protein antibody\|Coiled-coil myosin-like BCL2-interacting protein antibody\|GT197 antibody\|Protein GT197 antibody\|VPS 30 antibody\|VPS30 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Mouse, Rat Human	AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Beclin 1 antibody, ASA-B0216--1.jpg
IHC(P): Mouse Intestine Tissue

Anti-Beclin 1 antibody, ASA-B0216--2.jpg
IHC(P): Mouse Spleen Tissue

Anti-Beclin 1 antibody, ASA-B0216--3.jpg
IHC(P): Rat Intestine Tissue

Beclin 1 Antibody

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification

Immunogen

Properties

Form

Size

Contents

Concentration

Storage

Additional Information Regarding the Antigen

Gene

Protein

Uniprot ID

Function

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

Application Details