Storage
|
Store the unopened product at 2 - 8° C. Protect expiration date. from light. Do not use past |
Gene ID
|
281246 |
Gene Symbol
|
IL-10 |
Synonym
|
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF |
Species
|
Bovine |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of bovine IL-10. No significant cross-reactivity or interference between bovine IL-10 and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
31.3 pg/ml - 2000 pg/ml |
Sensitivity
|
25 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Bovine IL-10 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signaling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively. |