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Storage
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Store the unopened product at 2 - 8° C. Protect expiration date. from light. Do not use past |
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Gene ID
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100848466 |
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Gene Symbol
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NR3C3 |
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Synonym
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NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR |
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Species
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Bovine |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of bovine Total Progesterone R/NR3C3. No significant cross-reactivity or interference between bovine Total Progesterone R/NR3C3 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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12.5 pg/ml - 800 pg/ml |
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Sensitivity
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10 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Bovine Total Progesterone R/NR3C3 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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The progesterone receptor (PR), also known as NR3C3 or nuclear receptor subfamily 3, group C, member 3, is a protein found inside cells. It is activated by the steroid hormone progesterone. In humans, PR is encoded by a single PGR gene residing on chromosome 11q22, it has two main forms, PR-A and PR-B, that differ in their molecular weight. A third, lesser-known isoform, the PR-C, also exists. The PR-B is the positive regulator of the effects of progesterone, while PR-A and PR-C serve to antagonize the effects of PR-B. |
