Bovine Total Progesterone R/NR3C3 ELISA Kit

Catalog Number: AYQ-E10752
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect expiration date. from light. Do not use past
Gene ID 100848466
Gene Symbol NR3C3
Synonym NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR
Species Bovine
Specificity This assay has high sensitivity and excellent specificity for detection of bovine Total Progesterone R/NR3C3. No significant cross-reactivity or interference between bovine Total Progesterone R/NR3C3 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 12.5 pg/ml - 800 pg/ml
Sensitivity 10 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Bovine Total Progesterone R/NR3C3 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background The progesterone receptor (PR), also known as NR3C3 or nuclear receptor subfamily 3, group C, member 3, is a protein found inside cells. It is activated by the steroid hormone progesterone. In humans, PR is encoded by a single PGR gene residing on chromosome 11q22, it has two main forms, PR-A and PR-B, that differ in their molecular weight. A third, lesser-known isoform, the PR-C, also exists. The PR-B is the positive regulator of the effects of progesterone, while PR-A and PR-C serve to antagonize the effects of PR-B.